DETERMINATION OF PRILOCAINE HCl IN PHARMACEUTICAL PREPARATION AND HUMAN PLASMA WITH SPECTROPHOTOMETERY

A simple and sensitive spectrophotometric method was developed for the determination of prilocaine HCl in pharmaceutical preparation and human plasma. The quantitative analysis of prilocaine HCl was carried out using of wavelength at 230 nm. The method was linear in concentration range of 3-15 μg/mL for standard solution and 4-15 μg/mL for human plasma. Linearity was determined by calculating correlation coefficient. These values were found as a 0.9999 and 0.9967 in standard solution and human plasma, respectively. Developed spectrophotometric method was found suitable in terms of accuracy, sensitivity, precision, reproducibility. In addition to these, this method could be easily and directly applied to both human plasma and pharmaceutical preparation. INTRODUCTION: The reason of using local anesthetics is to block the conduction of impulses in nerve fibers which cause anesthesia. They are most commonly used in dentistry and minor surgery in order to provide temporary relief of pain. There are two application types of local anesthetics which are topical and parenteral applications that reversibly block the nerve conductances. Overdose of local anesthetics are toxic, causing cardiovascular and central-nervous disorders, and because of that reason therapeutic bloodplasma/serum concentrations should be kept in a certain concentration range. Similar to many other drugs, local anesthetics bind to plasma proteins to some extent. α1-acid glycoprotein and albumin . Prilocaine HCl, 2-propilamino-N-o-tolilpropiyonamit hydrochloride, is a drug. The chemical structure of prilocaine HCl is shown in Figure 1. FIG. 1: CHEMICAL STRUCTURE OF PRILOCAINE HCl Prilocaine is metobolyzed in liver. Prilocaine is hydrolyzed to hydrolyse to o-toluidine (OT) and Npropylalanine by the help of amidases enzymes. The main reason of methaemoglobinemia is taking overdose of prilocaine (exceeding 600 mg in adults or 8 mg kg in children) which increases the concentration of o-toluidine . In newborn infants, this enzyme, methaemoglobin, is not complete its development. Therefore, determination of prilocaine is clinically important for those patients. In these patients, less vasodilatation is clinically observed which is similar to other amide local anesthetics in terms of relative freedom from allergic reactions. For cardiac patients, It received short procedures which is administered in a plain solution .